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KMID : 1200020200440010193
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Diabetes & Metabolism Journal
2020 Volume.44 No. 1 p.193 ~ p.198
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A Novel Pancreatic Imaging Window for Stabilized Longitudinal In Vivo Observation of Pancreatic Islets in Murine Model
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Park In-Won
Hong Su-Jung
Hwang Yoon-Ha
Kim Pil-Han
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Abstract
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Longitudinal imaging of murine pancreas is technically challenging due to the mechanical softness of the tissue influenced by peristalsis. Here, we report a novel pancreatic imaging window for long-term stabilized cellular-level observation of the islets in the pancreas in vivo. By spatially separating the pancreas from the bowel movement and physiologic respiration with a metal plate integrated in the imaging window, we successfully tracked the pancreatic islets up to three weeks and visualized the dumbbell-shape transformation from the single islet. This window can be a useful tool for long-term cellular-level visualization of the microstructure in the pancreas.
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KEYWORD
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Intravital microscopy, Islets of Langerhans, Mice, Pancreas
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